Molecular Investigation of mrkA in Klebsiella pneumoniae Isolated from Nosocomial and Community Infection in Diyala Province, Iraq

Klebsiella pneumoniae is a pathogen relevant to hospital acquired and community such as urinary tract infections, pneumonia, wound burn. It has high levels of antibiotic resistance capability produce biofilm. This study included comparing among six patterns for the detected biofilm gene (mrkA). Clinical samples were collected from Teach Bagubah Hospital, Al-Batool Maternity Children Teaching private laboratories in Baqubah/Diyala, Iraq. To investigate antibiotics susceptibility, Kirby-Bauer disk diffusion method was used compare between isolates isolates. Hospital K. less susceptible than Both rates β-lactam/β -lactamase (Amoxicillin-clavulanate Ticarcillin- clavulante). Screening confirmation based on ESBL chromogenic agar are more sensitive producers double synergy test, which 30% 60% produce- ESBLs nosocomial acquired, 20% 40% respectively. The results gel electrophoresis PCR product using specific primers mrkA prevalence 83.33% 66.6% infection, demonstrated rest paper.